Journal: bioRxiv

Article Title: Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells

doi: 10.64898/2026.03.23.710523

Figure Lengend Snippet: (a) FSPs (FSP1 and FSP2) integrate probe and docking site sequences from Speed-optimized DNA-PAINT probe SP with the fluorophore-quencher design from Fluorogenic DNA-PAINT probe FP. Grey letters indicate locations of single-nucleotide mismatches in FP. (b) Bleaching effect in DNA-PAINT imaging under widefield illumination (yellow area) with regular and fluorogenic probes. (c) DNA-PAINT imaging of 20-nm grid DNA origami demonstrating that SP loses its ability to resolve the grid structures going from TIRF to widefield, but FSPs can resolve the structure under widefield illumination ( Suppl. Fig. 3b ). Scale bars: 20 nm. (d-g) Comparison of binding kinetics (k on and 1/k off ), localization precision, and speed value measured on DNA origami (n=3 independent experiments for each probe, each value averaged over ∼2000 origami) (see Suppl. Fig. 3d-h for more). (h) Overall DNA-PAINT probe comparison.

Article Snippet: The Dragonfly was used in the BTIRF mode, applying an objective-type TIRF or widefield configuration with an oil-immersion objective (Nikon Instruments, Apo SR TIRF 60×, NA 1.49, Oil).

Techniques: Imaging, Comparison, Binding Assay

Journal: Redox Biology

Article Title: S-nitrosylation of HINT1 in macrophages aggravates foam cell formation and atherosclerosis

doi: 10.1016/j.redox.2026.104063

Figure Lengend Snippet: S-nitrosylation of HINT1 aggravates ox-LDL induced foam cell formation through upregulating scavenger receptor A1 and CD36. (A) Representative images and quantification of oil red O staining in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). Scale bar: 50 μm. (B) Representative images and quantification of lipid droplet staining in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). Scale bar: 5 μm. (C) Total cholesterol level in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). (D) Representative images and quantification of Dil-ox-LDL uptake in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without Dil-ox-LDL (40 μg/mL) ( n = 6). Scale bar: 20 μm. (E) Cholesterol efflux to HDL was evaluated in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A and treated with or without ox-LDL (100 μg/mL) ( n = 6). (F) Western blot analysis of SR-A1, CD36 and LOX-1 in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). (G) Western blot analysis of SR-B1, ABCA1 and ABCG1 in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). (H) qPCR analysis of SR-A1, CD36 and LOX-1 in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). (I) qPCR analysis of SR-B1, ABCA1 and ABCG1 in HINT1 KO BMDMs transfected with Lenti-WT or Lenti-C84A followed by treatment with or without ox-LDL (100 μg/mL) ( n = 6). Data are presented as mean ± SEM. One-way ANOVA was used in A–C and E–I. Unpaired t -test was used in D.

Article Snippet: Antibodys against CD36 (18836-1-AP), SR-B1 (21277-1-AP), ABCA1 (26564-1-AP), ABCG1 (13578-1-AP), USF-2 (16614-1-AP), GAPDH (60004-1-lg), β-actin (66009-1-lg), and Tubulin (11224-1-AP) for western blotting, His-tag (66005-1-lg), Flag-tag (20543-1-AP), and HA-tag (51064-2-AP; 66006-2-lg) for western blotting and immunoprecipitation assay were obtained from Proteintech (Chicago, IL, USA); antibody against HINT1 (sc-271790), SR-A1 (sc-166184) for immunoprecipitation assay and immunofluorescence, CD36 (sc-7309) for immunofluorescence, USF2 (sc-293443) for immunoprecipitation assay were obtained from Santa Cruz Biotechnology (CA, USA).

Techniques: Staining, Transfection, Western Blot